Exploring environmental and pathogenic microbial diversity within our community

A metagenomics approach was used to take a closer look at bacterial communities living in sand samples taken from a local Bay area. In particular, we were interested in determining the abundance and diversity of pathogenic and biodegrading bacterial species within these samples. Metagenomics is a process used to identify mixed communities of microorganisms. Oxford
Nanopore sequencing technology showed there were diverse bacterial populations within each sequenced sample. Prior to sequencing, PCR was used to amplify 16S rDNA from samples to specifically identify bacteria. Post base-calling analysis of sequences using EPI2ME software revealed that samples contained pathogenic and biodegradable bacterial species. Studying
environmental microbial communities allows a closer look at microbial diversity within specific environments and the unique characteristics of these organisms that allow them to coexist, as well as their overall impact on the local environment.
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Abstract/Description: A metagenomics approach was used to take a closer look at bacterial communities living in sand samples taken from a local Bay area. In particular, we were interested in determining the abundance and diversity of pathogenic and biodegrading bacterial species within these samples. Metagenomics is a process used to identify mixed communities of microorganisms. Oxford Nanopore sequencing technology showed there were diverse bacterial populations within each sequenced sample. Prior to sequencing, PCR was used to amplify 16S rDNA from samples to specifically identify bacteria. Post base-calling analysis of sequences using EPI2ME software revealed that samples contained pathogenic and biodegradable bacterial species. Studying environmental microbial communities allows a closer look at microbial diversity within specific environments and the unique characteristics of these organisms that allow them to coexist, as well as their overall impact on the local environment.
Subject(s): Metagenomics
Undergraduate Research
Date Issued: 2021